Pure Cultures Lab Essay Example for Free

Pure Cultures Lab EssayIntroduction Pure market-gardenings atomic number 18 made of only one type of organisms and can be apply to study their properties. A system used to isolate pure cultures is making a steak- home office, which is a dilution process in which culture is air over an agar plate in a certain manner. Using a loop rod, culture was taken from the tube and dragged across area 1 several time,of the agar. The agar was then turned 90, and the loop was flamed and cooled. Taking some culture from area 1, it was dragged over area two,and the same steps were through with(p) for areas 3 and 4. Another technique used was spread-plate, where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod.The rod was dipped in 95% ethyl radical alcohol and flamed to sterlize. The nutrient agar was then placed on the plate, and spread with rod. An environmental plate was used to test the cultures of a random object, in our experiment, it was the oc ular lens of a microscope. A cotton dab was dipped into sterile water, and a random item of our choice was swabbed. After mixing the swab O.K. in the water, the contaminated water was applied to a spread plate.Results See attachedDiscussion e truly the plates were successful is isolating the pure cultures except the environmental. The reason for this may have been that there was no bacteria, delinquent to the fact they had been recently cleaned. The slant agars were able to pick up on the bacteria to show the growth. The phial that had bright yellow bacteria growing was M.leuteus, showing the successful isolation and identification. Other vials that had M.Letues and S.marcescenes had a very slight shade of bacteria growth.Questions1. No because a when a broth culture is used, it has not been inoculated from a pure culture, the only way would be to use a streaking method or spread plate. A mix culture slant is hard to isolate, because bacteria is clumped together, getting a sin gle colony is difficult. These may cause contamination to the bacteria during the inoculation period.2. If there was more culture in quadrant 4 than 3, it is due to the loop being dragged seat into quadrant 1. The nutrient agar that was in 1 came back to 4, and showed more culture.